DETERMINATION OF ORGANIC ACIDS IN GRAPE LEAVES USING REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Determination of ginkgolic acids from Ginkgo biloba leaves by reversed-phase argentation high performance liquid chromatography.

An analytical method has been firstly achieved for the quantification of ginkgolic acids from Ginkgo biloba leaves by reversed-phase argentation high performance liquid chromatography. Analytical sample was cleaned-up after addition of acidic salt solution and adsorbent to the matrix solution by counter-extraction of analytes with hexane. Ginkgolic acids were determined by HPLC with methanol an...

Reversed-phase high-performance liquid chromatography.

Analysis of Fatty Acids in Mouse Cells Using Reversed-Phase High-Performance Liquid Chromatography

A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to analyze various fatty acids in recombinant mouse L cells. These fatty acids were the metabolites of oleic acid. A process was developed to extract fatty acids from the cell samples before RP-HPLC analysis. The samples were first saponified w~th 0.5 M NaOH in 96% ethanol then extracted with acidified ethyl ...

Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography

Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was proposed. After pretreatment by dilution, centrifugation and filt...

Determination of Free Urinary Cortisol in Cushing’s Syndrome Using Reversed-phase High Performance Liquid Chromatography

Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing’s syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18 column, water-acetonitrile (72:28; v/v), with a flow rate of...